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KMID : 0382619900100010451
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Hanyang Journal of Medicine
1990 Volume.10 No. 1 p.451 ~ p.460
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Isolation and Characterization of Ribonucleases and Proteins Specific to Bladder Cancer
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Abstract
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Proteins and ribonuclease (RNase) were separated and fractionated by DEAE-cellulose column chromatography, high performance liquid chromatography(HPLC) and polyacrylamide gel electrophoresis(PAGE) to investigate proteins and RNases specific to bladder cancer. The properties of RNase specific to the bladder cancer were studied using homopolyribonucleotides and heteropolynucleotides as substrates.
(1) The protein was significantly increased by 16% and the activity of RNase was unchanged in bladder cancer tissues.
(2) Proteins in the bladder cancer tissue were separated by a DEAE-cellulose column chromatography by 7 peaks, of which 3 peaks exhibited RNase activity and 3 other non-RNase protein peaks appeared to be specific to the bladder cancer. Two protein peaks (one RNase peak and one non-RNase protein peak) normally present disappeared in the bladder cancer tissues.
(3) DEAE-cellulose protein peaks Ⅳ, Ⅴ and Ⅵ isolated from the bladder cancer tissue were further separated by HPLC into 5 to 6 subpeaks each, of which two of non-RNase protein subpeaks appeared to be specific to the bladder cancer. One of RNase subpeak normally present disappeared in the bladder cancer tissues.
(4) Analyses of DEAE-cellulose protein peaks Ⅳ, Ⅴ and Ⅵ from the bladder cancer tissue by native and SDS PAGE revealed that cancer spcific proteins were present in multiple in the bladder cancer tissue and that several protein bands present in the control bladder tissues disappeared in the bladder cancer tissues.
Results obtained in the present study indicated that RNase were present in the bladder cancer tissue in multiple as isozyme form, that RNase and non-RNase proteins specific to the bladder cancer appeared and that several RNase and non-RNase proteins present in the control tissue disappeared, activated or suppressed in the bladder cancer tissues. It could be suggested that changes in separation pattern of RNase and non-RNase proteins in the bladder cancer tissue were much more useful for understanding the nature of the cancer than changes in total protein contents and total activity of RNase.
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KEYWORD
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